SERCA1 ATPase Monoclonal Antibody (IIH11)

* CHF, excl. 8.1% VAT and shipping costs

Product Description

MA3-911 detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase from canine, human, rabbit, rat, mouse and guinea pig tissues. MA3-911 has been successfully used in Western blot, immunohistochemistry, and immunofluorescence procedures. By Western blot, this antibody detects an ~110 kDa protein representing SERCA1 ATPase in canine skeletal muscle extracts. Immunofluorescence staining of SERCA1 ATPase in canine skeletal muscle with MA3-911 results in strong labeling of the entire type II (fast) myofiber. The MA3-911 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum. This antibody recognizes an epitope between amino acids 199-505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum. ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expres