ApoA1 Monoclonal Antibody (6001)

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Product Description

By sandwich ELISA, MA5-14667 can be used as a detection antibody with Product # 710263 as the coating antibody, to generate a matched pair. Using this matched pair, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. It is not recommended to use Product # 701239 for coating when MA5-14667 is used for detection. By sandwich ELISA, MA5-14667 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MA5-14667, a biotinylated detection antibody followed by Streptavidin-HRP is recommended. By Western blot, MA5-14667 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MA5-14667 may not be as successful for detecting endogenous Apo A-1 by Western blot. Product MA514667 is a smaller package size of MIA1401 (formerly sold as a Seradyn product). Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.