EGFR Monoclonal Antibody (EGFR1), PE
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA528544 | 100 Tests | 335,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
The mouse monoclonal antibody EGFR1 reacts with extracellular domain of human protein kinase EGFR (ErbB1 / HER1); epitope within amino acids 6-273. Recommended dilution is for 100 µL of whole blood or 1x10^6 cells in suspension. EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Immunogen | Human epidermoid carcinoma line A431 |
Host | Ms |
Clone Number | [EGFR1] |
Genebank | EGFR |
Isotype | IgG2b,k |
Crossreactivity | Ho, Hu |
Clonality | Mab |
Format | liquid; PBS; pH 7.4, with 0.2% BSA and 15mM sodium azide |
Working Dilution | Flow Cytometry (10 µL/1x10^6 cells) |
Purity | Size-exclusion chromatography |
Conjugation | PE |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: MA5-28544 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |