HSF1 Monoclonal Antibody (4B4), PerCP
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA545714 | 100 µg | 406,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DN
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, ICC, IHC, IP, WB |
Regulatory Status | RUO |
Immunogen | Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439 |
Host | Rt |
Clone Number | [4B4] |
Genebank | HSF1 |
Isotype | IgG1 |
Crossreactivity | Bo, Gp, Hm, Hu, Monkey, Ms, Rb, Rt |
Clonality | Mab |
Format | Liquid; 2.15mM MES, 47.35mM phosphate; pH 7.4, with 1.033mM EDTA and no preservative |
Concentration | 1 mg/mL |
Working Dilution | ELISA (Assay-dependent), Gel Shift (Assay-dependent), Immunocytochemistry (1:100), Immunohistoche... |
Purity | Protein G |
Conjugation | PerCP |
Storage Conditions | 4° C |
Disclaimer | Alternative CatNo: MA5-45714 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |