IFN gamma Monoclonal Antibody (XMG1.2), Biotin

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Product Description

MM700B targets IFN gamma in ELISA, IHC-F, Neutralization and WB applications and shows reactivity with mouse and human samples. The MM700B immunogen is recombinant mouse IFN gamma. MM700B detects IFN gamma which has a predicted molecular weight of approximately 16 kDa. The MM700B mouse GMCSF antibody (biotinylated conjugate of clone XMG1.2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM700 (same clone). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. Biotinylated antibody MM700B (clone XMG1.2) and antibody MM700 (same clone) have successfully been used in combination with recombinant mouse IFNg protein SMIFNg in ELISA applications. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified. IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid