Leptin Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA1051 | 50 µL | 630,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
PA1-051 detects leptin from human, mouse, sheep and rat samples. PA1-051 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry experiments. By Western blot, this antibody detects an ~16 kDa protein representing leptin from 3T3-L1 cell extract. Immunohistochemical staining of leptin in sheep brain with PA1-051 results in staining of the ventromedial hypothalamus. The PA1-051 immunogen is a synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin. This sequence is completely conserved between mouse and bovine. Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated throu
Category | Antibody |
Supplier | ThermoFisher |
Application | ChIP, ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | Synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin. |
Host | Rb |
Genebank | LEP |
Isotype | IgG |
Crossreactivity | Hu, Ms, Sh, Rt |
Clonality | Pab |
Format | Liquid; PBS; , with 1mg/mL BSA and 0.05% sodium azide |
Concentration | 2 mg/mL |
Working Dilution | Immunohistochemistry (Frozen) (1:50 - 1:200), Immunocytochemistry (2 µg/mL), Western Blot (1:200-... |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: PA1-051 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |