HMGB1 Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA116926 | 100 µL | 604,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody is expected to cross react with porcine, equine and rabbit samples based on 100% sequence homology. Suggested positive control: Hela whole cell extract, antigen standard for HMGB1 (transient overexpression lysate). HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. HM
Category | Antibody |
Supplier | ThermoFisher |
Application | ChIP, EL, FC, ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | Synthetic peptide between to amino acids 100-200 of the human HMGB1 protein. |
Host | Rb |
Genebank | HMGB1 |
Isotype | IgG |
Crossreactivity | Bo, Dg, Hu, Ms, Sh, Rb, Rt |
Clonality | Pab |
Format | Liquid; tris glycine; , with 0.02% sodium azide |
Concentration | 1 mg/mL |
Working Dilution | Western Blot (0.5-1 µg/mL), Immunohistochemistry (Paraffin) (1:100-1:250), Immunocytochemistry (0... |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | 4° C, do not freeze |
Disclaimer | Alternative CatNo: PA1-16926 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |