LC3A/LC3B Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA116931 | 100 µL | 638,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody detects both LC3A and LC3B.Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Recommended positive controls: LC3B overexpression lysate, mouse brain whole tissue lysate (adult whole normal), human brain whole tissue lysate (adult whole normal), Neuro2a whole cell lysate, Neuro2a Chloroquine-treated/untreated cell lysate, HeLa chloroquine-treated/untreated cell lysate This antibody is predicted to react with Bovine based on 100% sequence homology. Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homo
Category | Antibody |
Supplier | ThermoFisher |
Application | ChIP, EL, FC, ICC, IHC, IP, WB |
Regulatory Status | RUO |
Immunogen | Synthetic peptide corresponding to a region between residues 25-121 of human LC3 protein. |
Host | Rb |
Genebank | MAP1LC3A |
Isotype | IgG |
Crossreactivity | Dg, Fish, Hu, Ms, Plant, Rt, Zebrafish |
Clonality | Pab |
Format | Liquid; PBS; , with 0.02% sodium azide |
Concentration | 1 mg/mL |
Working Dilution | ChIP Assay (Assay-Dependent), ELISA (Assay-Dependent), Flow Cytometry (Assay-Dependent), Western ... |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | -20°C or -80°C if preferred |
Disclaimer | Alternative CatNo: PA1-16931 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |