IL5RA (soluble) Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA124792 | 25 µg | 606,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Recommended positive controls: human blood-derived granulocytes. The Neutralization Dose50 (ND50) for anti-human IL5RA is approximately 1-3 µg/mL in the presence of 0.5 ng/mL of recombinant human IL-5 using the human TF-1 cell line. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cell surface IL5RA mediated IL5 response on a responsive cell line, at a specific IL5 concentration. The exact concentration of antibody required to neutralize the human cell surface IL5RA mediated bioactivity is dependent on the IL5 concentration as well as on the number of IL5RA present on the cell surface (a function of the cell type and culture conditions). Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cell replacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membrane-proximal proline-r
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, N, WB |
Regulatory Status | RUO |
Immunogen | Purified, recombinant human interleukin 5 soluble receptor alpha (IL5RA) expressed in insect Sf 21 cells. |
Host | Gt |
Genebank | IL5RA |
Isotype | IgG |
Crossreactivity | Hu |
Clonality | Pab |
Format | Liquid; PBS; pH 7.4, with 5% trehelose and no preservative |
Concentration | 0.1 mg/mL |
Working Dilution | Flow Cytometry (2.5 µg /10^6cells), Inhibition Assays (1-3 µg/mL), Neutralization (1-3 µg/mL), We... |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Disclaimer | Alternative CatNo: PA1-24792 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |