Osteopontin Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA125152 | 25 µg | 658,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
By Western Blot, a doublet band of approximately 65 kDa and a band of 30 kDa is observed due to glycosylation. A suggested positive control is 293 cells. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/mL in the presence of 2 µg/mL of recombinant mouse osteopontin using 293 cells. Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, IHC, N, WB |
Regulatory Status | RUO |
Immunogen | Recombinant, full length mouse protein, expressed in NSO cells. |
Host | Gt |
Genebank | SPP1 |
Isotype | IgG |
Crossreactivity | Ms |
Clonality | Pab |
Format | Liquid; PBS; , with 5% trehalose and no preservative |
Concentration | 0.1 mg/mL |
Working Dilution | Immunohistochemistry (5-15 µg/mL), ELISA (0.2-0.8 µg/mL), Western Blot (0.1-0.2 µg/mL), Inhibitio... |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Disclaimer | Alternative CatNo: PA1-25152 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |