5-Methylcytosine Polyclonal Antibody
| Catalog Number | Pack Size | List Price* | Quantity | |
|---|---|---|---|---|
| PA130675 | 250 µg | 852,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
The use of 7mM NaOH in the IP is crutial. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
| Category | Antibody |
| Supplier | ThermoFisher |
| Application | DB, FC, ICC, IHC, IP, WB |
| Regulatory Status | RUO |
| Immunogen | 5-methyl cytosine molecule conjugated to KLH. |
| Host | Sh |
| Isotype | IgG |
| Crossreactivity | Chemical |
| Clonality | Pab |
| Format | Liquid; 0.15M PBS; , with no preservative |
| Concentration | 3 mg/mL |
| Working Dilution | Flow Cytometry (Assay-dependent), Immunocytochemistry (5 µg/mL), Immunoprecipitation (25-100 µg/m... |
| Purity | purified |
| Conjugation | Unconjugated |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Disclaimer | Alternative CatNo: PA1-30675 |
| Tech. Data Sheet | View datasheet |
| Link To Supplier | https://www.thermofisher.com |
| Material Safety Data Sheet | Download PDF |
