TPH1 Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA14678 | 100 µL | 650,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody is predicted to react with wide variety of mammals based on 100% sequence homology, however it does not recognize TPH in rabbit tissues. This antibody is specific for the ~55 kDa TPH protein in Western blots of human dorsal Raphe nucleus. Immunolabeling has been demonstrated in rat brain extracts. Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
Category | Antibody |
Supplier | ThermoFisher |
Application | IHC, WB |
Regulatory Status | RUO |
Immunogen | Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE |
Host | Sh |
Genebank | TPH1 |
Isotype | IgG |
Crossreactivity | Hu, Rb, Rt |
Clonality | Pab |
Format | Liquid; 0.01M HEPES; pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol and no preservative |
Working Dilution | Immunohistochemistry (1:1,000), Immunohistochemistry (Frozen) (1:1,000), Western Blot (1:1,000) |
Purity | Affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: PA1-4678 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |