HSP90 alpha Polyclonal Antibody

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Product Description

PA3-013 detects heat shock protein 90 (HSP90) from human, monkey, mouse, sheep (adrenal) and rat tissues and cells. This antibody does not detect HSP84. PA3-013 has been successfully used in Western blot, immunofluorescence, immunohistochemistry (paraffin) and immunoprecipitation procedures. By Western blot, this antibody detects an ~86 kDa protein representing HSP86 from HeLa cell lysate. Immunofluorescence staining of HSP86 in HeLa cells with PA3-013 yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP86 exists primarily as homodimers in HeLa cells. Furthermore, the antibody is capable of precipitating HSP86 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor. The PA3-013 immunogen is a synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence. Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be ass