Phospho-RNA pol II CTD (Ser2, Ser5) Polyclonal Antibody
| Catalog Number | Pack Size | List Price* | Quantity | |
|---|---|---|---|---|
| PA517563 | 100 µL | 602,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
It is not recommended to aliquot this antibody. RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
| Category | Antibody |
| Supplier | ThermoFisher |
| Application | ChIP, IHC, WB |
| Regulatory Status | RUO |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer2/5 of the Rpb1 CTD heptapeptide |
| Host | Rb |
| Genebank | POLR2A |
| Isotype | IgG |
| Crossreactivity | Hm, Hu, Ms, Rt, Yeast |
| Clonality | Pab |
| Format | Liquid; 0.01M HEPES; pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol and no preservative |
| Working Dilution | Immunohistochemistry (Paraffin) (1:25), Western Blot (1:1,000), ChIP Assay (1-3 µL) |
| Purity | Antigen affinity chromatography |
| Conjugation | Unconjugated |
| Storage Conditions | -20°C |
| Disclaimer | Alternative CatNo: PA5-17563 |
| Tech. Data Sheet | View datasheet |
| Link To Supplier | https://www.thermofisher.com |
| Material Safety Data Sheet | Download PDF |
