Cripto Polyclonal Antibody, DyLight™ 550
| Catalog Number | Pack Size | List Price* | Quantity | |
|---|---|---|---|---|
| PA522702 | 100 µL | 606,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
In Western blot a band is seen at ~18 kDa representing the core form of Cripto1, and at ~36 kDa reporesenting the glycosylated/ post-translationally modified form. Suggested positive control: MDA-MB231 (human breast cancer) lysates. Cripto (TDGF1) is a GPI-anchored cell membrane proetin involved in Nodal signaling. Cell associated TDGF1 acts as a a Nodeal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulated Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. TDGF1 is expressed both in ES cells and during the early phases of embryo development, while in adults it is reactivated in a wide range of epithelial cancers.
| Category | Antibody |
| Supplier | ThermoFisher |
| Application | FC, ICC, WB |
| Regulatory Status | RUO |
| Immunogen | A synthetic peptide made to an N-terminal portion of the murine cripto protein sequence (between residues 1-50). |
| Host | Rb |
| Genebank | TDGF1 |
| Isotype | IgG |
| Crossreactivity | Hu, Ms |
| Clonality | Pab |
| Format | Liquid; 50mM sodium borate; , with no preservative |
| Concentration | 0.94 mg/mL |
| Working Dilution | Immunocytochemistry (1:1,000), Western Blot (2 µg/mL), Flow Cytometry (Assay-Dependent) |
| Purity | Antigen affinity chromatography |
| Conjugation | DY 550 |
| Storage Conditions | 4° C, store in dark |
| Disclaimer | Alternative CatNo: PA5-22702 |
| Tech. Data Sheet | View datasheet |
| Link To Supplier | https://www.thermofisher.com |
| Material Safety Data Sheet | Download PDF |
