HMGB1 Polyclonal Antibody, DyLight™ 650
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA522720 | 100 µL | 660,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody will not work in ICC/IF applications. HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. HMGB1 is highly conserved and ubiquitous in the nuclei and cytoplasm of nearly all cell types, is a necessary and sufficient mediator of inflammation during sterile and infection-
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, FC, ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). |
Host | Rb |
Genebank | HMGB1 |
Isotype | IgG |
Crossreactivity | Bo, Dg, Hu, Ms, Sh, Rb, Rt |
Clonality | Pab |
Format | Liquid; 50mM sodium borate; , with 0.05% sodium azide |
Concentration | 0.76 mg/mL |
Working Dilution | Flow Cytometry (Assay-Dependent), Immunocytochemistry (Assay-Dependent), ELISA (Assay-Dependent),... |
Purity | Antigen affinity chromatography |
Conjugation | DY 650 |
Storage Conditions | 4° C, store in dark |
Disclaimer | Alternative CatNo: PA5-22720 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |