IRE1 alpha Polyclonal Antibody, DyLight™ 650
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA522725 | 100 µL | 606,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody does not react with non-human primate samples. Suggested positive control: Pancreatic islets and insulinoma cells. IRE1 alpha is the endoplasmic reticulum to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. IRE1 alpha protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.
Category | Antibody |
Supplier | ThermoFisher |
Application | ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | A synthetic peptide within the human IRE1 alpha protein (within residues 700-800). |
Host | Rb |
Genebank | ERN1 |
Isotype | IgG |
Crossreactivity | Hu, Ms, Rt |
Clonality | Pab |
Format | Liquid; 50mM sodium borate; , with no preservative |
Concentration | 1 mg/mL |
Working Dilution | Western Blot (1:1,000-1:2,000), Immunocytochemistry (Assay-Dependent), Immunohistochemistry (Para... |
Purity | Antigen affinity chromatography |
Conjugation | DY 650 |
Storage Conditions | 4° C, store in dark |
Disclaimer | Alternative CatNo: PA5-22725 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |