HIF1A Polyclonal Antibody, DyLight™ 550
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA522780 | 100 µL | 652,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody detects upregulation of HIF-1 alpha in hypoxic samples. HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is loca
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein. |
Host | Rb |
Genebank | HIF1A |
Isotype | IgG |
Crossreactivity | Dg, Fish, Gt, Hm, Hu, Ms, Primate, Pg, Rb, Rt |
Clonality | Pab |
Format | Liquid; 50mM sodium borate; , with 0.05% sodium azide |
Concentration | 0.84 mg/mL |
Working Dilution | Immunohistochemistry (Frozen) (5-10 µg/mL), Immunohistochemistry (Paraffin) (5-10 µg/mL), Western... |
Purity | Antigen affinity chromatography |
Conjugation | DY 550 |
Storage Conditions | 4° C, store in dark |
Disclaimer | Alternative CatNo: PA5-22780 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |