LYVE1 Polyclonal Antibody, DyLight™ 550
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA522784 | 100 µL | 662,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Suggested positive control: mouse spleen lysate. LYVE1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module, the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. LYVE1 is a type I integral membrane glycoprotein. LYVE-1 is expressed primarily on lymphatic vessel endothelium and is likely to be involved in regulating the traffic of leucocytes and tumor cells to lymph nodes. The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environmen
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | A synthetic peptide made to a C-terminal portion of the murine LYVE-1 protein sequence (between residues 250-318). |
Host | Rb |
Genebank | LYVE1 |
Isotype | IgG |
Crossreactivity | Hu, Ms, Rt |
Clonality | Pab |
Format | Liquid; 50mM sodium borate; , with 0.05% sodium azide |
Concentration | 0.66 mg/mL |
Working Dilution | Flow Cytometry (1:25), Western Blot (1:500-1:2,000), Immunocytochemistry (Assay-Dependent), Immun... |
Purity | Antigen affinity chromatography |
Conjugation | DY 550 |
Storage Conditions | 4° C, store in dark |
Disclaimer | Alternative CatNo: PA5-22784 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |