ATG5 Polyclonal Antibody, DyLight™ 488
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA522856 | 100 µL | 652,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
In Western Blot, a band is seen ~56 kDa representing the ATG5-ATG12 complex, the molecular weight of human ATG5 is ~33 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Suggested positive control: mouse wildtype ES cell lysate. ATG5 (Autophagy Related 5) is an important element for autophagy and may play an important role in the apoptotic process. ATG5 is also involved in other cellular processes that include mitochondrial quality control after oxidative damage, negative regulation of the innate anti-viral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. ATG5 contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5-ATG12 conjugate forms a cup-shaped isolation membrane that then detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may play a role in the apoptotic process, possibly within the modified cytoskeleton. Further, APG5 expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. The
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, FC, ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | A synthetic peptide made to an N-terminal region (within residues 1-50) of the human ATG5 protein. |
Host | Rb |
Genebank | ATG5 |
Isotype | IgG |
Crossreactivity | Bo, Drosophila, Fish, Gp, Hu, Ms, Primate, Pg, Rt, Reptile, Xenopus, Zebrafish |
Clonality | Pab |
Format | Liquid; 50mM sodium borate; , with 0.05% sodium azide |
Concentration | 0.81 mg/mL |
Working Dilution | Western Blot (1:500), Immunocytochemistry (Assay-Dependent), ELISA (Assay-Dependent), Flow Cytome... |
Purity | Antigen affinity chromatography |
Conjugation | DY 488 |
Storage Conditions | 4° C, store in dark |
Disclaimer | Alternative CatNo: PA5-22856 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |