H3K27me3 Polyclonal Antibody
| Catalog Number | Pack Size | List Price* | Quantity | |
|---|---|---|---|---|
| PA531817 | 100 µL | 668,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Recommended positive controls: 293T, A431, HeLa, HepG2, Jurkat, Raji, K562, THP-1, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. H3K27me3 is an epigenetic silencing mark and thus associated with downregulation of nearby genes and often found in heterochromatin regions. H3K27me3 is mainly found at inactive gene promoters and generally is found in opposition to the activating mark H3K4me3. H3K27me3 often occupies gene rich regions with close association to development regulators. EZH2, a component of the PRC2 complex, is the histone methyltransferase (or writer) responsible for trimethylating H3K27. UTX and JMJD3 both are histone demethylases (or erasers) for H3K27me3. The regulation of H3K27me3 is crucial for gene regulation and dysregulation of H3K27me3 has been implicated in numerous diseases including cancer. H3K27me3 antibodies are often used for western blot and ChIP applications to study changes in levels and occupancy of H3K27me3.
| Category | Antibody |
| Supplier | ThermoFisher |
| Application | DB, ICC, IHC, WB |
| Regulatory Status | RUO |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 22 and 36 of Human Histone H3 |
| Host | Rb |
| Genebank | H3C1 |
| Isotype | IgG |
| Crossreactivity | Hu, Ms, Rt, Zebrafish |
| Clonality | Pab |
| Format | Liquid; PBS; pH 7, with 1% BSA, 20% glycerol and 0.025% ProClin 300 |
| Concentration | 1 mg/mL |
| Working Dilution | Western Blot (1:500-1:3,000), Immunohistochemistry (Paraffin) (1:100-1:1,000), Immunocytochemistr... |
| Purity | Antigen affinity chromatography |
| Conjugation | Unconjugated |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Disclaimer | Alternative CatNo: PA5-31817 |
| Tech. Data Sheet | View datasheet |
| Link To Supplier | https://www.thermofisher.com |
| Material Safety Data Sheet | Download PDF |
