HDAC1 Polyclonal Antibody
| Catalog Number | Pack Size | List Price* | Quantity | |
|---|---|---|---|---|
| PA532443 | 500 µL | 598,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil or breast carcinoma. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.
| Category | Antibody |
| Supplier | ThermoFisher |
| Application | ChIP, ICC, IHC, WB |
| Regulatory Status | RUO |
| Research Field | PROTEIN & CELL ANALYSIS |
| Immunogen | Synthetic peptide derived from C-terminus of human HDAC-1 protein |
| Host | Rb |
| Genebank | HDAC1 |
| Isotype | IgG |
| Crossreactivity | Hu, Ms |
| Clonality | Pab |
| Format | Liquid; PBS; pH 7.6, with 1% BSA and 0.1% sodium azide |
| Working Dilution | Western Blot (1:1,000), Immunocytochemistry (1:100), ChIP Assay (2.5 µg/10^6 cells), Immunohistoc... |
| Purity | Antigen affinity chromatography |
| Conjugation | Unconjugated |
| Storage Conditions | Freezer (-5 to -30 C) |
| Disclaimer | Alternative CatNo: PA5-32443 |
| Tech. Data Sheet | View datasheet |
| Link To Supplier | https://www.thermofisher.com |
| Shipping Details | Ambient |
| Material Safety Data Sheet | Download PDF |
