PARP1 Polyclonal Antibody
| Catalog Number | Pack Size | List Price* | Quantity | |
|---|---|---|---|---|
| PA534803 | 100 µL | 754,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Recommended positive controls: 293T, A431, HeLa, HepG2, HCT116, HCT116 (30 µM cisplatin treatment for 24 hr), NIH-3T3, PC-12, H1299. Predicted reactivity: Mouse (90%), Rat (88%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
| Category | Antibody |
| Supplier | ThermoFisher |
| Application | ChIP, ICC, IHC, IP, WB |
| Regulatory Status | RUO |
| Research Field | PROTEIN & CELL ANALYSIS |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 334 and 619 of PARP1 (Uniprot ID#P09874) |
| Host | Rb |
| Genebank | PARP1 |
| Isotype | IgG |
| Crossreactivity | Hu, Ms, Rt |
| Clonality | Pab |
| Format | Liquid; PBS; pH 7, with 1% BSA, 20% glycerol and 0.025% ProClin 300 |
| Concentration | 0.28 mg/mL |
| Working Dilution | ChIP Assay (Assay-Dependent), Western Blot (1:500-1:20,000), Immunocytochemistry (1:100-1:1,000),... |
| Purity | Antigen affinity chromatography |
| Conjugation | Unconjugated |
| Storage Conditions | Freezer (-5 to -30 C) |
| Disclaimer | Alternative CatNo: PA5-34803 |
| Tech. Data Sheet | View datasheet |
| Link To Supplier | https://www.thermofisher.com |
| Shipping Details | Wet Ice |
| Material Safety Data Sheet | Download PDF |
