IL-1 beta Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA547204 | 100 µg | 604,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human IL-1 beta, recombinant mouse IL-1 beta, and recombinant canine IL-1 beta is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method. Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta a
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, ICC, N, WB |
Regulatory Status | RUO |
Immunogen | E. coli-derived recombinant feline IL-1 beta/IL-1F2 Ala116-Ser267 |
Host | Gt |
Genebank | IL1B |
Isotype | IgG |
Crossreactivity | Ca |
Clonality | Pab |
Format | Lyophilized; PBS; , with 5% trehalose and No Preservative |
Concentration | 0.2 mg/mL |
Working Dilution | ELISA (0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)), Immunocytochemistry (5-15 µg/mL), Neu... |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: PA5-47204 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |