FAS Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA547283 | 100 µg | 580,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Fas is observed. Reconstitute at 0.2 mg/mL in sterile PBS. FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominant
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, ICC, WB |
Regulatory Status | RUO |
Immunogen | Mouse myeloma cell line NS0-derived recombinant feline Fas/TNFRSF6/CD95 Ala25-Lys172 |
Host | Gt |
Genebank | FAS |
Isotype | IgG |
Crossreactivity | Ca |
Clonality | Pab |
Format | Lyophilized; PBS; , with 5% trehalose and No Preservative |
Concentration | 0.2 mg/mL |
Working Dilution | Flow Cytometry (2.5 µg per million cells), Immunocytochemistry (5-15 µg/mL), Western Blot (0.1 µg... |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: PA5-47283 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |