IL-1 beta Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA547480 | 100 µg | 602,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
In direct ELISAs, approximately 100% cross-reactivity with recombinant canine IL-1 beta, recombinant feline IL-1 beta, and recombinant human IL-1 beta is observed, 40% cross-reactivity with recombinant porcine IL-1 beta, recombinant mouse IL-1 beta, and recombinant rat IL-1 beta is observed, and 10% cross-reactivity with recombinant cotton rat IL-1 beta and is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method. Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its ac
Category | Antibody |
Supplier | ThermoFisher |
Application | ICC, N, WB |
Regulatory Status | RUO |
Immunogen | E. coli-derived recombinant equine IL-1 beta/IL-1F2 Ala116-Ala268 (Glu179Gly, Met188Thr, Thr194Ile, Ser245Lys, Arg256Gln) |
Host | Gt |
Genebank | IL1B |
Isotype | IgG |
Crossreactivity | Ho |
Clonality | Pab |
Format | Lyophilized; PBS; , with 5% trehalose and No Preservative |
Concentration | 0.2 mg/mL |
Working Dilution | Immunocytochemistry (20-35 µg/mL), Neutralization (0.05-0.25 µg/mL), Western Blot (0.1 µg/mL) |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: PA5-47480 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |