IL-12 p70 Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA547961 | 100 µg | 550,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-12 is observed, and less than 5% cross-reactivity with recombinant mouse IL-12 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method. Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct fro
Category | Antibody |
Supplier | ThermoFisher |
Application | N, WB |
Regulatory Status | RUO |
Immunogen | CHO-derived recombinant porcine IL-12 p35/p40 heterodimer Ile23-Asn324 (p40), Arg26-Ser222 (p35) |
Host | Gt |
Genebank | IL12A |
Isotype | IgG |
Crossreactivity | Pg |
Clonality | Pab |
Format | Lyophilized; PBS; , with 5% trehalose and No Preservative |
Concentration | 0.2 mg/mL |
Working Dilution | Neutralization (0.01-0.04 µg/mL), Western Blot (0.1 µg/mL) |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: PA5-47961 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |