C. botulinum BoNT-C1 Heavy Chain Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
PA548078 | 100 µg | 606,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant (r) BoNT-D-HCR is observed and 5% cross-reactivity with rBoNT-A-HCR, rBoNT-A-LC, and rBoNT-B-LC is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum. BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain. BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction. E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.
Category | Antibody |
Supplier | ThermoFisher |
Application | IP, WB |
Regulatory Status | RUO |
Immunogen | E. coli-derived recombinant BoNT-C1 Heavy Chain Asn866-Glu1291 |
Host | Sh |
Isotype | IgG |
Crossreactivity | Bacteria |
Clonality | Pab |
Format | Lyophilized; PBS; , with 5% trehalose and No Preservative |
Concentration | 0.2 mg/mL |
Working Dilution | Immunoprecipitation (25 µg/mL), Western Blot (0.1 µg/mL) |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: PA5-48078 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |