HIF1A Polyclonal Antibody

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Product Description

Keep as concentrated solution. Predicted reactivity: Mouse (90%), Rat (90%), Dog (97%), Pig (95%), Rabbit (95%), Bovine (97%). Positive Control: MCF-7, HeLa(500uM CoCl for 24hr), NIH-3T3 (500 uM CoCl2 treatment for 24 hr), HepG2 whole cell lysate/extract (1% O2 treatment for 24hr), 293T transfected HIF1A, HepG2 ( 200 uM CoCl2 treatment for 24 hr), HepG2 (500 uM CoCl2 treatment for 24 hr). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor a