Phospho-Histone H3 (Ser10) Polyclonal Antibody

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Product Description

Keep as concentrated solution. Positive Control: HeLa, HeLa (100 ng/ml Nacodazole treatment for 24 hr), U2OS(100ng/ml Nocodazole treatment for 24hr), 4T1 (100ng/ml Nocodazole treatment for 24hr), Rat2 (100 ng/ml Nocodazole treatment for 24 hr). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.