6x-His Tag Monoclonal Antibody (3D5), AP
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
R93225 | 125 µL | 516,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
NOTE: R932-25 was previously sold under product #46-0284. When re-ordering, please use #R932-25. R932-25 detects 6x His tag (C-term) -His-His-His-His-His-His-COOH. Detects fusion proteins containing this (requires the free carboxyl group for detection (Lindner et al., 1997). This product contains enough material for 25 western blots. Western blot experiments with 100 ng of recombinant Positope™ control protein gave a detectable signal within 15 minutes using a chromogenic substrate. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5. For Western blot, do not dilute the AP-conjugated antibody into PBS. Phosphate inhibits alkaline phosphatase. We recommend diluting the AP-conjugated antibody in Tris-Buffered Saline (TBS) containing 0.1% (v/v) Tween-20 and 1% (w/v) nonfat dry milk. If you use a different buffer for washing and blocking your blots, then dilute as described above with that buffer. You may use other blocking agents such as bovine serum albumin (BSA) or gelatin. All AP-conjugated antibodies have been tested in immunoblotting procedures. Low background was observed. No cross-reactivity has been observed in bacterial lysates. The 6x His tag is a synthetic oligo peptide
Category | Antibody |
Supplier | ThermoFisher |
Application | WB |
Regulatory Status | RUO |
Immunogen | 6x His synthetic peptide: C-term -His-His-His-His-His-His-COOH |
Host | Ms |
Clone Number | [3D5] |
Isotype | IgG2b |
Crossreactivity | Tag |
Clonality | Mab |
Format | Liquid; PBS; , with 0.01% sodium azide |
Concentration | 1-2 mg/mL |
Working Dilution | Western Blot (1:1,000-1:5,000) |
Purity | purified |
Conjugation | AP |
Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Disclaimer | Alternative CatNo: R932-25 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |