V5 Tag Monoclonal Antibody, AP
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
R96225 | 125 µL | 590,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
NOTE: R962-25 was previously sold under product #46-0287. When re-ordering, please use #R962-25. R962-25 recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-. This product contains enough material for 25 Western blots. Western blot experiments with 100 ng of recombinant Positope™ control protein gave a detectable signal within 15 minutes using a chromogenic substrate. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5. For Western blot, we recommend diluting the AP-conjugated antibody in Tris-Buffered Saline (TBS) containing 0.1% (v/v) Tween-20 and 1% (w/v) nonfat dry milk. In Western blot applications, no cross-reactivity has been observed in bacterial lysates. Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKP
Category | Antibody |
Supplier | ThermoFisher |
Application | WB |
Regulatory Status | RUO |
Immunogen | V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-. |
Host | Ms |
Isotype | IgG2a |
Crossreactivity | Tag |
Clonality | Mab |
Format | Liquid; PBS; , with 0.01% sodium azide |
Concentration | 1-2 mg/mL |
Working Dilution | Western Blot (1:1,000-1:5,000) |
Purity | purified |
Conjugation | AP |
Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Disclaimer | Alternative CatNo: R962-25 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |