Goat anti-Rabbit IgG Fc, Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
A78954 | 500 µg | 296,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This goat anti-rabbit IgG Fc secondary antibody has been affinity-purified and shows minimum cross-reactivity to human, mouse and rat serum proteins. These antibodies have been solid phase adsorbed to ensure class specificity and cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondary antibodies flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g. flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 555 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 555 nm laser line. For stable si
Category | Antibody |
Supplier | ThermoFisher |
Application | Secondary antibody, FC, ICC, IHC, WB |
Regulatory Status | RUO |
Species Specificity | Rb |
Immunogen | Gamma Immunoglobin Fc region |
Host | Gt |
Crossreactivity | Rb |
Clonality | Pab |
Format | Liquid; PBS; pH 7.5, with 5mM sodium azide |
Concentration | 2 mg/mL |
Working Dilution | Flow Cytometry (2-8 µg/mL), Immunocytochemistry (1-2 µg/mL), Immunohistochemistry (Assay-dependen... |
Purity | purified |
Conjugation | Alexa Fluor 555 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |