Zymo-Seq SPLAT DNA Library Kit

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Product Description

The Zymo-Seq™ SPLAT DNA Library Kit is a versatile solution for DNA library preparation from a wide variety of sample types. By applying the unique, single-stranded library preparation approach of Splinted Ligation Adapter Tagging (SPLAT), DNA libraries can be prepared from genomic DNA, cell-free DNA, and even FFPE-derived DNA with ease. Additionally, the SPLAT technology allows for direct ligation of adapters onto the native ends of each DNA fragment, simultaneously eliminating the need for end-repair and ensuring preservation of all original nucleotides. This feature allows for accurate and high-fidelity analysis of fragmentation patterns, setting a new standard for fragmentomics analysis.The Zymo-Seq™ SPLAT DNA Library Kit easily generates library from 10 ng to 500 ng of pre-fragmented DNA input in just two simple steps: (a) ligation of the single-stranded DNA with the unique splinted adapters concurrently and (b) amplification of the libraries via PCR using unique dual indexes. By utilizing a straightforward workflow that is efficient and user-friendly, the Zymo-Seq™ SPLAT DNA Library Kit allows for generation of high-quality libraries ready for DNA sequencing in as little as 3 hours.