ELISA kit for human Fc protein

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Product Description

EK000095-HUFC: ELISA kit for human Fc proteinApplication: for the quantitative determination of human Fc proteins in cell culture supernatants and serum.This assay is specific for human Fc, and does not cross react with mouse Fc.Kit components for 96-well plate ELISA for human Fc protein (2x 96-well microplates):1 Pre-coated Microplate: 96 well polystyrene microplates coated with polyclonal antibody specific for human Fc protein; 2 plates2 Detection Antibody: a horseradish peroxidase (HRP)- conjugated polyclonal antibody against human Fc protein; 1 vial (20 ul)3 Human Fc Protein Standard: 10,000 ng/ml human Fc protein; 1 vial (50 ul)4 Assay Diluent: 1x PBS with 1% BSA; 1 bottle (60 ml)5 Wash Buffer: 10x Concentrate- PBS with detergent; 1 bottle (60 ml)6 Color Reagent: tetramethylbenzidine (TMB); 1 bottle (20 ml)7 Stop Solution: 0.5N hydrochloric acid solution; 1 bottle (20 ml)8 Product Insert: product description, assay protocol, material data sheet; 1 book.BackgroundFc is the tail region of an immunoglobulin G (IgG) that interacts with cell surface receptors called Fc receptors and some proteins of the complement system. The ~230 amino acid fragment generally exists as a dimer, although under reduced condition, it exists as a monomer. It is the basis of prolonge